Theyll give your presentations a professional, memorable appearance the kind of sophisticated look that todays audiences expect. The pink color from gram negative bacteria comes from a counter stain of safranin. We applied this method to forty two archived gram stained slides. Gram stain report introduction the purpose of this experiment is to determine the shape and gram stain of the bacteria under a microscope. This differential staining procedure separates most bacteria into two groups on the basis of cell wall composition. Introduction gram staining is the most essential and universally used staining technique in bacteriology laboratory.
This method is used to distinguish between gram positive and gramnegative bacteria which have. It includes introduction, history,principle, method of gram staining procedure slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising. You will be asked about differential staining, grampositive and. The staining technique, developed in the late 1700s by christian gram classifies the rigid cell walled bacteria into one of two groups based on whether they are able to resist the decolorizing action of an alcoholic. Gramstaining was firstly introduced by cristian gram in 1883. Gramstain mohammad rahbar reporting 4 issue a written preliminary report with the gram s stain results 5 telephonically notify the ordering provider or ward of. Gram staining is a very important technique used in biology labs all over the world. Jan 15, 2020 flagella are too thin to be visualized using a bright field microscope with ordinary stains, such as the gram stain, or a simple stain. Some staining techniques, such as the gram and ziel neelsen stains, although of great diagnostic value.
These organisms are usually filamentous, gram positive rods of varying length that often form chains, but occasionally, they may stain gram negative. Gram staining free download as powerpoint presentation. Gram stain control slide provides two airdried and methanolfixed droplets within two etched circles. The gram stain is a classical and important staining technique that remains widely used by. Ppt gram positive bacteria powerpoint presentation free. Sop on gram staining objective to lay down the procedure for gram staining.
Gram staining lab report essay sample sample essays. The gram stain is a type of differential stain that allows a microbiologist to. National public health laboratory world health organization. Responsibility microbiologist or above of microbiology laboratory. Add the primary stain crystal violet and incubate 1 minute. Gram staining a important technique a staining technique used to classify bacteria. It includes introduction, history,principle, method of gram staining procedure. Overheating of smears when heat fixation is used d. The most widely used staining procedure in microbiology is the gram stain, discovered by the danish scientist and physician hans christian joachim gram in 1884. Pdf gram staining is an empirical method of differentiating bacterial species into two large groups grampositive. Students can use this activity as a prelab or to supplement a unit on bacteria and pathology. Basic microbiology lab reporting gram staining free download as powerpoint presentation. Prepare the smear of suspension on the clean slide with a loopful of sample. The gram stain is used to differentiate bacteria into two groups based on the cell color after staining.
A wet mount technique is used for staining bacterial flagella, and it is simple and useful when the number and arrangement of flagella are critical to the identification of species of motile bacteria. This staining procedure differentiates microbes into two basic groups. If the staining procedure is performed correctly, gram positive bacteria will be purple, while gram negative bacteria will be pink. Christian gram in 1884, and categorizes bacteria according to their gram character gram positive or gram negative. Ppt gram stain powerpoint presentation free to view. Record the qc results on the gram stain qc sheet provided in annex 1. To prepare the bacteria culture slides, a volume of 1 ml of bacterial. You will be asked about differential staining, gram positive and. Make a slide of tissue or body fluid that is to be stained. Bacterial characteristics gram staining video khan academy. Worlds best powerpoint templates crystalgraphics offers more powerpoint templates than anyone else in the world, with over 4 million to choose from. In the first step, the nuclei are stained with a hematoxylin solution. In view of this phenomenon, gram dried a smear from a lung sample and poured. Oct 24, 2019 both gram positive and gram negative bacteria pick up the pink stain, but it is not visible over the darker purple of the gram positive bacteria.
It is one of the differential stains that are used to characterize bacteria in one of two groups. In 1883 hans christian gram discovered an important differential staining method that is used extensively today. Although simple stains are useful, they do not reveal details about the bacteria other than morphology and arrangement. The circle nearest the label contains a droplet of gram positive staphylococcus aureus derived from atcc 25923. Gram staining in diagnostic microbiology slideshare. Endospores resist adverse environmental conditions such as dryness, heat and poor nutrient supply. Beyer individual supplies microscope slide clothes pin inoculating loop or wood splint paper towels shared supplies crystal violet stain safranin red stain grams iodine ethyl alcohol denatured water bottle rinsing basin alcohol burner wax pencil matches microscope create a staining area on your microscope slide obtain a clean microscope slide draw a. Our new crystalgraphics chart and diagram slides for powerpoint is a collection of over impressively designed datadriven chart and editable diagram s guaranteed to impress any audience. For confirmation of bacterial presence and identity, we used standard staining including gram and acidfast staining 33, 34.
Winner of the standing ovation award for best powerpoint templates from presentations magazine. Heat the slide for few seconds until it becomes hot to the touch so that bacteria are firmly mounted to the slide. The acid fast stain was discovered by ehrlich, who found that after staining with. Apr 04, 2016 a staining technique used to classify bacteria. Gram staining is a differential staining technique that differentiates bacteria into two groups. Screening of the quality of the clinical specimens such as sputum that should contain many.
It is a technique used to differentiate types of bacteria using certain physical and chemical characteristics of their cell walls. In gram staining the purple color in gram positive bacteria comes from a crystal violet dye. The gram stain is a differential staining technique used to classify. Flagella stain principle, procedure and result interpretation. Ppt microbiology powerpoint presentation free to download. Crystal violet primary stain for 1 minrinse gram s iodine mordant for 1 minrinse acetonealcohol decolorizer for 5 seconds safranin counterstain for 1 minrinse gram negative red and gram positive blue assess gram reaction a. Gram staining is an essential procedure used in the identification of bacteria and is frequently the only method required for studying their morphology. These online assessment tools will help you strengthen your understanding of the gram stain method. Gram staining is still the cornerstone of bacterial identification and taxonomic division. Bacteria study web in this activity, students will use resources found on the web to gain an understanding of how bacteria are stained and classified.
The word gram is always spelled with a capital, referring to hans christian gram, the inventor of gram staining gram staining principles gram staining is used to determine gram status to classify bacteria broadly. Based on differences in cell wall components, bacteria are categorized as either gram positive stains dark purple or gram negative stains pink. Before staining it is essential to fix the bacterial sample on to the slide. Times new roman arial wingdings default design slide 1 key words prokaryotes prokaryotes bacteria eukaryotes prokaryotic cell versus eukaryotic cell bacteria versus archaebacteria bacteria versus archaebacteria slide 9 bacteria slide 11 slide 12 slide outer membrane gram negative cell envelope gram positive cell envelope flagella slide. Affordable and search from millions of royalty free images, photos and vectors. Was working with respiratory disease in lung tissue from cadaver at municipal hospital berlin and his accidental spillage of lugols iodine.
Biology ppts videos microbiology mcqs microbiology notes. Gram staining method, the most important procedure in microbiology, was developed by danish physician hans christian gram in 1884. Selection of empirical antibiotics based on gram stain finding. Scope this sop is applicable for gram staining in microbiology laboratory of pharmaceutical company name. Historical background hans christian joachim gram, danish bacteriologist and physician. Selection of suitable culture media based on gram stain finding. Developed gram staining technique in 1883 and published his findings in 1884 in friedlanders journal. Crystal violet dye is added for one minute to stain the cell wall. In addition this stain also allows determination of cell morphology, size, and arrangement. Requirements for gram staining technique glass slides 25. Gram stain the previous lab introduced simple staining techniques that enable microbiologists to observe the morphological characteristics of bacteria. Gram staining of bacteria from environmental sources.
The microscopicanalysis using gram staining technique showed that majority of the bacterial isolates were grampositive bacteria while only three of. Chart and diagram slides for powerpoint beautifully designed chart and diagram s for powerpoint with visually stunning graphics and animation effects. Gram staining of bacteria from environmental sources protocol. The circle farthest from the label contains a droplet of a gram negative escherichia coli derived from atcc 25922. Gram staining method, the most important procedure in microbiology, was. Ppt gram stain procedure powerpoint presentation free. Pdf lab report of microbiology 3 akbar haqi academia. For gram positive staphylococcus aureus is used, for gram negative e. Rapid presumptive diagnosis of diseases such as bacterial meningitis. The gram stain is the most frequently used stain in a clinical microbiology laboratory and is usually the first step in identifying bacteria. Simple in shape, but genetically and biochemically advanced. Principle, procedure, interpretation, examples and animation.
Jan 08, 2020 the gram staining technique is the most important and widely used microbiological differential staining technique. The gram stain virtual interactive bacteriology laboratory. A free powerpoint ppt presentation displayed as a flash slide show on id. Principle, procedure, interpretation, examples and.
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